Mast launches new test for VTEC detection

Scientific Company News

Mast has announced the launch of Mast Isoplex VTEC, a new test that uses loop-mediated isothermal amplification to detect verotoxin-producing E. coli (VTEC).

The test offers numerous advantages over traditional culture methods by offering the ability to detect all strains that express vtx1 and vtx2, with samples prepared in a simplified 15-minute boiling step, eliminating the need for expensive and time-consuming extraction processes.

“Mast has introduced its new Mast Isoplex VTEC test, a means of identifying one of the major causes of foodborne illness in humans.“

It includes a detection dye that provides visual colour detection, allowing results to be generated within an hour; meanwhile, the availability of 48-test and 96-test formats allows laboratories to scale their screening capabilities according to demand.

The Scottish E.coli O157/VTEC Reference Laboratory (SERL) recently carried out an evaluation of Mast Isoplex VTEC and issued a positive verdict, stating it is "a useful adjunct to current VTEC testing at diagnostic laboratories that do not have access to PCR testing".

VTEC is one of the major causes of foodborne illness in humans, particularly the VTEC-O157 strain, which is transmitted when food becomes contaminated with the faeces of infected animals.

A recent outbreak of VTEC-0157  in the UK was linked to salad leaves and cheese, resulting in more than 150 cases of infection and two deaths.

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