Integrated DNA Technologies has published the latest instalment of its quarterly newsletter Decoded, which includes an in-depth discussion of the science behind big DNA.
The article has been provided by Dr Dan Gibson, an expert in the field of synthetic biology who has spent much of the last decade developing advances in this field with the J Craig Venter Institute and Synthetic Genomics.
“Integrated DNA Technologies has published its latest quarterly newsletter, which includes a discussion of the science of big DNA by expert Dr Dan Gibson.“
A means of taking synthetic biology beyond its current limitations, understanding of big DNA techniques has been propelled by the work of Dr Gibson, who in 2010 produced a DNA construct of 1.1 Mb, a feat that was not attainable using traditional restriction/ligation methodologies.
He is responsible for the development of the Gibson Assembly Method, which uses a unique enzyme mix to assemble DNA elements with 20 to 80 overlapping bases, making the population - once reamplified and enriched with the correct sequence - much faster to screen.
Dr Gibson is now looking to refine DNA assembly methods further by improving large gene delivery to host cells and creating a means of ending dependence on host organisms for copying their synthetic constructs.
This comes after Integrated DNA Technologies attended MipTec 2013 last week, where representatives gave a talk on rapid and reliable gene construction with gBlocks gene fragments and the Gibson Assembly Method.See all the latest jobs in Science