Scipio Bioscience Produce New Asteria Single-cell RNA-sequence Kit with Accompanying Cytonaut Cloud Software

Laboratory

Now commercially available, Scipio Bioscience have created an innovative benchtop kit that facilitates the sequencing of 10,000 mammalian single cells per sample. Alongside the laboratory materials, researchers have access to their cloud-based analysis software, Cytonaut, facilitation bioinformatic analysis of transcriptomic data rapidly, and for researchers of all abilities. This software analyses output data, generates interactive data visualisation, results and figures that are ready for inclusion in research papers set for publication. This can be done from anywhere, permitting remote analysis away from the laboratory, and also provides sample quality indicators, sequence quality controls, and generates trajectory analysis of the data to inform next steps.

This kit provides rapid turnover analysis of single-cell datasets. The kit is low-cost, and due to its effective stable stopping point of 2 hours with a further 5 stopping points throughout the experimental process, meaning experiments can take place for the maximum amount of time during the working day, and may be continued later without the degradation of cellular or genetic material. This makes it much more efficient than many existing kits used in commercial laboratories, and and the incorporation of all necessary components into one kit, makes for a great investment in all high-throughput laboratories. Furthermore, the protocol requires only basic laboratory techniques, meaning all abilities and resource-settings may make use of the Asteria kit, and processing of samples can happen rapidly, preserving sample integrity and producing data more closely representative of physiological or experimental conditions in the target cells.

“Scipio Bioscience Produce New Asteria Single-cell RNA-sequence Kit with Accompanying Cytonaut Cloud Software“

The kit is designed using a hydrogel platform, and includes a cell-labelling component with a bead-specific DNA barcode for capturing genetic material, a poly-T sequence for mRNA polyA tail capture, diluents, gelification materials, cell lysis factors, and RT and PCR amplification cDNA solutions, producing samples ready for sequencing. The Asteria kit is compatible with NEBNext ® Ultra II FS DNA Library Prep kit from New England Biolabs and the Illumina Nextera XT kit, although Scipio are intending to validate further library preparation kits, meaning it can be easily incorporated into existing protocols without extra expenditure or new skillsets.

Current research projects utilising the Asteria kit include elucidating the molecular pathophysiology of Parkinson’s disease, the genetic basis for nervous system tumours, and the transcriptomics of metabolic diseases of the pancreas (https://scipio.bio/resources/).

https://www.labbulletin.com/articles/the-asteria-singlecell-rnaseq-kit-and-the-cytonaut?utm_source=feedgrabbr&utm_content=In_the_news

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