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Chromatography is a method of separation. A sample dissolved in a "mobile phase" is passed through a stationary phase (the chromatography column) to separate the analyte. The mobile phase maybe a liquid or a gas sample that needs to be separated, plus a solvent which moves the sample through the chromatography column. The data output is a chromatography, which shows peaks and troughs to correspond to the different components of the separated sample. Techniques of chromatography include gas chromatography (GC), liquid chromatography (LC, HPLC), thin layer chromatography (TLC), size-exclusion, supercritical fluid chromatography (SFC), and gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). |
